摘  要：核黄素对人体有许多重要的作用，研究核黄素的结构有助于人类理解多种疾病的发生与发展机理，并有望为新型药物的设计和应用提供理论指导。

传统的对于核黄素的检测方法有诸多的劣势，如需要真空环境、、灵敏度不够高、无法获得分子层次的信息、容易收到其他信号的干扰等。使用拉曼光谱分析法进行检测存在着操作简便，测定时间短，灵敏度高等优点。但是激光激发拉曼散射的同时会产生比较强的荧光，荧光的信号会完全覆盖拉曼散射的信号，从而我们检测不到真正的拉曼光谱。因此，要想获得比较好的拉曼散射信号必须要将荧光淬灭。

本文主要研究用表面增强拉曼光谱基底来进行荧光淬灭。首先通过掠入射沉积法，制备银纳米棒阵列表面增强拉曼散射光谱基底，并使用生物染料分子双酚A聚氧乙烯醚进行检测，确认其符合要求。接着以黄素单核苷酸为样品，使用制备好的表面增强拉曼基底对其进行检测。从检测结果来看，相比较拉曼光谱，表面增强拉曼光谱可以有效的使荧光淬灭。换用多种不同浓度的黄素单核苷酸样品进行重复实验，发现随着浓度的增大，信号会到达一个最大值然后下降。

通过实验我们证实表面增强拉曼光谱技术在研究荧光物质方面表现出了非常优良的特性。为未来的荧光淬灭和测量提供研究思路。

该论文有图10幅，表1个，参考文献50篇

关键词：核黄素  黄素单核苷酸  荧光淬灭  表面增强拉曼散射光谱  掠入射沉积法

Study on Fluorescence Quenching and Structure of Flavin Mononucleotide

Abstract：Riboflavin has many important roles in human body. The study of the structure of riboflavin helps to understand the mechanism of the occurrence and development of various diseases, and is expected to provide theoretical guidance for the design and application of new drugs.

There are many disadvantages in the traditional detection methods for riboflavin, such as the need for vacuum environment, high sensitivity, inability to obtain information on the molecular level, and the interference of other signals easily. Raman spectroscopy has the advantages of simple operation, short detection time and high sensitivity. However, laser excitation of Raman scattering will produce relatively strong fluorescence, and the signal of the fluorescence will completely cover the signal of Raman scattering, thus we can not detect the real Raman spectrum. Therefore, if we want to get better Raman scattering signal, we must quenching the fluorescence.

In this paper, the fluorescence quenching of SERS substrate is studied. First, the surface enhanced Raman scattering (SERS) substrate of a silver nanorod array was prepared by the grazing incidence method, and the bio dye molecule bisphenol A polyoxyethylene ether was used to detect the substrate. Then the flavin mononucleotide was used as the sample, and the surface enhanced Raman substrate was used to detect it. From the test results, compared with Raman spectroscopy, surface enhanced Raman spectroscopy can effectively quench fluorescence. Repeated experiments were carried out with different concentrations of flavin mononucleotide samples. It was found that with the increase of concentration, the signal reached a maximum and then decreased.

Experiments show that SERS technology has shown excellent properties in the study of fluorescent materials. It provides a research idea for future fluorescence quenching and measurement.

The paper has 10 drawings, 1 tables and 50 references

Key Words: riboflavin  flavin mononucleotide  fluorescence quenching  surface enhanced Raman scattering spectroscopy  grazing incidence deposition

目  录

摘  要 I

Abstract II

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变量注释表 V