摘要：介孔二氧化硅微球具有有序规则的孔径和孔容，目前在药物的运输和释放领域有着极大的发展潜力。本课题先对癌症的治疗，纳米药物载体以及介孔二氧化硅的几种制备及测试方法进行了简单介绍，然后采用两种不同的模板剂：CTAB和P123分别与TEOS作为硅源进行反应，并且通过亲水性的聚乙二醇单甲醚（mPEG）修饰，得到具有高比表面积、高孔容积以及高孔隙率的SiO2-mPEG亲水微球。本课题通过电镜观察比较了微球的表面形貌，选择出结果较好的制备方案，并通过红外光谱仪分析了二氧化硅微球的化学基团，判断了修饰的成功与否。在这基础上，本课题选取了抗癌药物—阿霉素（DOX）作为模型药物，将其包埋于制备好的介孔二氧化硅微球中形成一个载药微囊，对比了mPEG修饰前后的微球中DOX的释放行为的变化。结果发现，经mPEG修饰的介孔二氧化硅微球具有更高的比表面积，更好的载药运输能力，为药物的缓释控制及靶向性提供了保障。

关键词： 药物运输；二氧化硅；mPEG修饰；纳米介孔材料；癌症

Study on the Drug Delivery And anti-tumor properties of mPEG-modified Mesoporous Silica

Abstract：Mesoporous silica microspheres have well-ordered pore size and pore volume, and currently have great potential for development in the field of drug delivery and release. In this topic, we briefly introduced some cancer treatment, nano drug carriers, and several methods for the preparation and testing of mesoporous silica. I then used two different templating agents: CTAB and P123, which reacted with TEOS respectively and were modified with hydrophilic mPEG. As the result,I obtained SiO2-mPEG hydrophilic microspheres with high specific surface area, high pore volume, and high porosity. In this study, the surface morphology of two kinds of microspheres was compared by electron microscopy, and a good preparation scheme was selected based on the results. The chemical groups of the silica microspheres were analyzed by infrared spectroscopy to determine the success or failure of the modification. On this basis, I chose the anticancer drug DOX as a model drug and embedded it in the prepared mesoporous silica microspheres to form a drug-loaded microcapsule. I compared the release behavior of DOX in microspheres before and after mPEG modification. The results showed that the mPEG-modified mesoporous silica microspheres have a higher specific surface area, better drug delivery capacity, and provide guarantees for sustained release control and targeting of drugs.

Key Words： Drug Delivery；Silica；mPEG modification; Nanoporous materials；Cancer

目  录

1 研究的背景、目的和意义 1

1.1 课题选择的背景及原因 1

1.2 介孔材料简介 3

2 文献综述 5

2.1 纳米药物载体的研究与进展 5

2.1.1 纳米磁性颗粒 5

2.1.2 纳米智能药物载体 5

2.1.3 树枝形聚合物 5

2.2 介孔二氧化硅的基本性质和合成机理 6

2.3 介孔二氧化硅的主要制备方法 6

2.3.1 溶胶-凝胶法 6

2.3.2 微乳法 6

2.3.3 水热法 6

2.3.4 微波法 7

3 测试与表征方法 8

3.1 MSNs的基础理化性能表征 8

3.1.1 MSNs 微球的微观形貌 8

3.1.2 红外光谱分析( FT-IR) 8

3.1.3 射线衍射分析( XRD) 8

3.1.4 BET 氮气吸附 8

3.2 微球 DOX 载药情况测试 8

3.3 DOX体外释放过程 9

3.4 抗癌效果测试 9

4 主要实验方法 10

4.1 主要实验药品及仪器 10

4.1.1 主要实验药品 10

4.1.2 主要实验仪器 10

4.2 介孔二氧化硅微球的制备 10

4.3 mPEG修饰 11

4.4 载药试验 12

4.5 释放试验 12

5 实验结果与讨论 13

5.1 二氧化硅微球的形貌 13

5.2 红外图谱解析 15

5.3 载药性能分析 17

5.4 释药性能分析 17