摘要：本实验以法国薰衣草作为研究材料，建立子叶和下胚轴为研究材料的愈伤组织再生体系,分析不同外植体消毒方法对薰衣草种子的萌发情况，观察子叶和下胚轴，在不同浓度配比的IAA、6-BA和IAA的培养下，其愈伤组织的形成和不定芽分化的情况，比较不同培养基下分化苗生根情况，筛选出薰衣草最佳消毒方法、子叶和下胚轴的出愈率和出芽率较高的培养基组合,最适合生根的培养基。

结果：实验发现,通过先将薰衣草种子温水浴40℃浸泡2h,常温后用高猛酸钾进行消毒30min，冲洗干净后用酒精浸泡90s，0.1%次氯酸钠消毒时间为15min的灭菌方法，种子存活率能达到85.3%，污染率最低,同时，经过温水浸泡的种子萌发率会有所提高。在MS培养基基础上添加不同的植物生长调节剂，当IAA为0.5mg/L，6-BA为2.0mg/L条件时，相对于其他浓度条件，子叶的出愈率和出芽率较高，分别为100%和26.7%，下胚轴在0.1mg/LIAA+1.0mg/L6-BA条件下不定芽分化效果达到最佳，其不定芽分化能力比子叶强，出芽率达到35.7%。当IAA浓度过高时，其不定芽分化能力会受到抑制。在1/2MS培养基，添加IAA1.0mg/L，分化苗生根情况最佳，根系生长正常，移栽苗都能成活。

关键词：薰衣草；萌发；愈伤组织；分化苗

Culture and bud differentiation of lavender Cotyledon and Hypocoty

Abstract: This experiment takes French lavender as the research material, the Cotyledon and hypocotyl were used to study the Callus regeneration system of the materials, and the germination of lavender seeds was analyzed by different explant disinfection methods, and the cotyledons and Hypocotyl were cultured in different concentrations of IAA, 6-BA and IAA,The Callus formation and adventitious bud differentiation, compared with different culture medium differentiated seedlings rooting situation, screening the best disinfection method of lavender, Cotyledon and hypocotyl of the higher rate and budding rate of the medium combination, the most suitable for rooting medium.

Results: The experiment found that through the first lavender seed water  bath  40  ℃ immersion 2h, Disinfection of 30 min with high potassium acid at normal temperature, rinse clean with alcohol soaked for 90s,0.1% sodium hypochlorite disinfection time for 15 min sterilization method, seed survival rate of 85.3%, the lowest pollution rates, at the same time, The germination rate of seeds was increased after soaking with warm water. With the addition of different plant growth regulators on the basis of MS Medium, when IAA was 0.5mg/l,6-ba as 2.0mg/l condition, the yield and budding rate of cotyledons were higher than other concentration conditions, respectively 100% and 26.7%, hypocotyl Under the condition of 0.1mg/l IAA and 1.0mg/l 6-BA, adventitious bud differentiation is the best, its adventitious bud differentiation ability is stronger and budding rate reaches 35.7%. When IAA concentration is too high, adventitious bud differentiation ability will be inhibited. In 1/2 MS Medium, IAA 1.0mg/l was added to differentiate seedling rooting, root growth was normal and transplant seedlings could survive.

Key words: lavender; germination;callus; differentiation seedlings

目 录

第一章绪论 1

1.1薰衣草的概述 1

1.1.1薰衣草的不同品种 1

1.1.2薰衣草的生活习性 3

1.1.3薰衣草的主要价值 3

1.1.4薰衣草的繁殖方法 3

1.2薰衣草组织培养的国内外相关研究发展 4

1.2.1外植体的处理研究 4

1.2.2种子萌发研究 5

1.2.2以芽、茎、叶等为外植体相关研究 5

1.3课题的目的和研究意义 6

第二章不同消毒方法对薰衣草种子的影响 6

2.1材料 7

2.2方法 7

2.3结果与分析 8

第三章薰衣草子叶与下胚轴分化培养 10

3.1材料 10

3.2方法 10

3.3结果与分析 10