1材料和方法

1.1材料来源

在美国生物数据库NCBI的Protein中下载得到了6种植物液泡转化酶的蛋白序列[4]，基因登录号分别是AAB68679.1、CAF22241.1、AIU48037.1、AFP23357.1、BAF35859.1、ABX55832.1，并获得了编码蛋白序列的mRNA序列，长度分别为2346bp、2038bp、2345bp、2292bp、2274bp、2178bp。蛋白序列如下：

菜豆AAB68679.1

MEHHKPLLPTSSHAAPNPRTRKDLLLLLCALLFLSSLVAFGRNRASNVPHDHVSSSASNHQQEHQSPTSLPSSKWHAVSRGVSSGVSEKSSSMLFSGEGGASEAFPWDNSMLSWQRTSFHFQPEKNWMNDPNGPMYYKGWYHFFYQYNPNGAVWGDIVWGHAVSRDMIHWLHLPLAMVADQWYDKQGVWTGSATILPNGEIIMLYTGSTNESVQVQNLAYPADPSDPLLVDWIKHPGNPVLVPPPGIGAKDFRDPTTAWLTSEGKWRITIGSKLNKTGIALVYDTDDFKTYELKNGHLRAVPGTGMWECVDFFPVSKKNENGLDTSLSINGAEVKYVMKVSLDDDRHDYYTIGTYDENKVLFTPDDVKNDVGVGLRYDYGIFYASKTFYDQNMDRRILWGWIGESDSEYADVTKGWASVQSIPRTVRLDKKTGSNLLQWPVAEVESLRLRSDEFKSLKAKPGSVVSLDIETATQLDVVAEFEIDAESLQKTAQSNEEFTCSTSGGAAQRGALGPFGLLVLADEGLSEYTPVYFYVIKGRNGNLKTSFCSDQSRSSQPNDVRKQIFGNIVPVLEGEKFSLRMLVDHSIVESFAQGGRTCVTSRVYPTKAIYGAARLFLFNNATEATVTASLKIWQMNSAFIRPFPFNPDQKN

大麦CAF22241.1

MPTMDTTDRGSYAQLPDDAEAGSAHRRRTGPLCAAILLTSAALLLAVAALAGVRVAGQLPVAGVIMSGQPTTVDVVPTTSTSSRGPEYGVSEKTSGAGAHGGMLGADAGNAFPWSNAMLQWQRTGFHFQPEKNWMNDPNGPVYYKGWYHLFYQYNPDGAIWGNKIAWGHAASRDLLRWRHLPVAMSPDQWYDINGVWSGSATVLPDGRIVMLYTGSTNASVQVQCLAFPTDPSDPLLINWTKYENNPVMYPPPGVGEKDFRDPTTAWFDGPDDMWRLVIGPKDDRHAGMVMTYKTKDFMDYELVPGLLHRVPGTGMWECIDLYPVGGVRGIDMTDAVTAASNNGGDDVLHVMKESSDDDRHDYYALGRYDATKNTWTPLDVDADLGIGLRYDWGKFYASKTFYDPAKKRRVLWGWVGETDSESADVAKGWASLQSTPRAVVLDTKTGSNLLQWPVEEVETLRTNSTDIGGVTIDRGSVFALNLHRATQLDIEASFRLDQLDIAASNEADVGYNCSTSGGAAGRGKLGPFGLLVLADARRYGGDAERTAVYFYVARGLDGGLHTHFCHDEMRSSHANDIVKRVVGNTVPVLDGEELSVRVLVDHSIVESFAMGGRLTATSRVYPTEAIYANAGVYLFNNATGIQVTTTRLVVHEMDSS

高粱AIU48037.1

METRDTTAPLPYSYTPLPAADAASAEVTGTGHRGGRSRRRSLCAAALVLSAALLLAVAALTGVGRRVDVVPGGAGSPRSTSSISRGPDAGVSEKTSGAWSGGGRLRSDGAGGNAFPWSNAMLQWQRTGFHFQPHMNWMNDPNGPVYYKGWYHLFYQYNPDGAIWGNKIAWGHAVSRDLIHWRHLPLAMVPDQWYDTNGVWTGSATTLPDGRLAMLYTGSTNASVQVQCLAVPADDADPLLTNWTKYEGNPVLYPPPGIGPKDFRDPTTAWFDPSDNTWRIVIGSKDDAEGDHAGIAVVYRTKDFVSFELLPGLLHRVARTGMWECIDFYPVATRGKASGNGVDMSDAFGKNGAIVGDVVHVMKASMDDDRHDYYALGRYDAATNEWTPLDAEKDVGIGLRYDWGKFYASKTFYDPAKRRRVLWGWVGETDSERADVSKGWASLQGIPRTVLLDTKTGSNLLQWPVEEAETLRTNSTDLSGITIDYGSAFPLNLRRATQLDIEAEFQLDRRAVMSLNEADVGYNCSTSGGAAARGALGPFGLLVLADKHLREQTAVYFYVAKGLDGSLTTHFCQDESRSSSANDIVKRVVGSSVPVLDDETTLSLRVLVDHSIVESFAQGGRSTATSRVYPTEAIYANAGVFLFNNATAARVTAKKLVVHEMDSSYNHDYMVTDI

荔枝AFP23357.1

MDTHNPSHLHAPYYTPLPEHPSTGGPPATLRRPLKGFALILASVIFLLSLVGLIIINQSRQPLQNSTSNVNIPSLLSPPPPSFSRRVPRGVEEGVSAKSNPSPFDQESSYNWTNAMFSWQRTSFHFQPTRNWMNDPDGPLYHMGWYHLFYQYNPDSAVWGNITWGHAVSRDLIHWLYLPIAMVPDQPYDINGVWTGSATLLPDGQIVMLYTGDTAELVQVQNLAYPANLSDPLLLHWVKYSGNPVLVPPTHIAPKDFRDPTTGWIGPDGKWRITIGSKINGTGFSLIYHTTDFKTYELLDHVLHAVPGTGMWECVDFYPVAINGSMGLDTSAGGPGIKHVLKASLDDTKLDHYALGTYDPDSDTWTPDNPEEDVGIGLRYDNGRYYASKTFYDQYRKRRILWGWINETDTEYDDLEKGWASVQTIPRTVLFDNKTGINIVQWPVQEVESLRQRSIEFEDVLIEPGSIVPLDIGTATQLDIFAEFETELLESAGEEEGYSCKGGAINRNKLGPFGLLVIADDSLSELTPVFFRPTNTTNGTLDTYFCTDETRSTLAPEVQKHIYGSTVPVLEGERYAMRVLVDHSIVESFAQGGRTVITSRIYPTEAIYGSARLFLFNNATGVNVKATLKIWQMNSAFIHPFPL

砂梨BAF35859.1

MADPRPFLPSDPDALPYTYTPLPNPDEASEIRKRQFKELLAVFSGLLMLSLLVVIISSGHNSDSHANKNEPVFLAKEPYTTRPAKLRPVSRGIAAGVSEKSNRLADAVDGPAFPWNNSMLSWQRTAFHFQPEKNWMNDPNGPLFYKGWYHFFYQWNPNGAVWGDIVWGHAVSKDLIHWLHLPLAMVADQWYDINGVWTGSATILPDGKIVMLYTGSTNESVQVQNLAYPADHNDPLLTKWVKYSGNPILVPPPGIGYKDFRDPTTAWYTSQGKWRITIGSKLNKTGISLVYDTKDFKTYEQLNGVLHAVPGTGMWECVDFYPVSKTSDKGLDTSVNGPDVKHVVKASLDDDRNDYYSLGSYEEKTGKWVPDNQKIDVGIGIRYDYGIFYASKTFYDQNKERRVLWGWIGESDSENADMQKGWASVQGIPRTVLFDKKTGSNLIQWPVEEVENLRLSITDFDKVEVKAGSVLPLQVVTATQLDIVAEFELDKKVLESVAESNEVFSCNTSAGGSHRGALGPFGLLVLADETLSEQTPVYFYVAKGPGGNFDTFFCADQTRSSVANDVKKKVTGSYVPVLKDEKLSVRILVDHSIVESFAQGGRTTITTRVYPTKAIYGAARLFLFNNATEISVTASLKVWQMNSAFIRPYHPDETSNASLNLVTYIKFIVTFFIFLTLSFLT

香瓜ABX55832.1

sMATQCYDPENSASRYTLLPDQPDSGHRKSLKIISGIFLSVFLLLSVAFFPILNNQSPDLQIDSRSPAPPSRGVSQGVSDKTFRDVAGASHVSYAWSNAMLSWQRTAYHFQPQKNWMNDPNGPLYHKGWYHLFYQYNPDSAIWGNITWGHAVSKDLIHWLYLPFAMVPDQWYDINGVWTGSATILPDGQIMMLYTGDTDDYVQVQNLAYPANLSDPLLLDWVKFKGNPVLVPPPGIGVKDFRDPTTAWTGPQNGQWLLTIGSKIGKTGVALVYETSNFTSFKLLDGVLHAVPGTGMWECVDFYPVSTKKTNGLDTSYNGPGVKHVLKASLDDNKQDHYAIGTYDLGKNKWTPDNPELDCGIGLRLDYGKYYASKTFYDPKKERRVLWGWIGETDSESADLQKGWASVQSIPRTVLYDKKTGTHLLQWPVEEIESLRVGDPTVKQVDLQSGSIELLRADSAAELDIEASFEVDKVALQGIIEADHVGFSCSTSGGAASRGILGPFGVIVIADQTLSELTPVYFYISKGADGHAETHFCADQTRSSEAPGVGKQVYGSSVPVLDGEKHSMRLLVDHSIVESFAQGGRTVITSRIYPTKAVNGAARLFVFNNATGASVTASVKIWSLESANIQSFPLQDL