摘要：本课题从甜橙油微乳液出发，通过单因素实验来确定甜橙油微乳液的最佳配方，运用分光光度计以及纳米粒度分析仪对制备出的微乳液进行透光率和粒径测定并比较，同时使用离心机测试微乳液样品的稳定性。然后将甜橙油微乳液加至卷烟中，并进行人工感官评吸；利用GC-MS分析卷烟烟气混合物。实验结果表明：最终确定甜橙油微乳液最佳配方为表面活性剂用量T-80:蔗糖月桂酸酯=9:1，表面活性剂和助表面活性剂间比例Km=3:1，平均粒径只有20.16nm，透光率在95%以上，经过离心后的微乳液仍保持均一稳定，未出现分层和浮油的现象；甜橙油直接添加和微乳液添加的卷烟样品间的感官属性具有显著差异；3种卷烟样品共检测出155种香气成分，在这些物质中占比较多的挥发性化合物是杂环类、酮类、酯类化合物。

关键词：甜橙油；微乳；卷烟；感官评定

Preparation and Application of Orange Oil Microemulsion

Abstract: This topic started from the sweet orange oil microemulsion and determined the best formula of the orange oil microemulsion through single factor experiment. The light transmittance and particle size of the prepared microemulsion are measured by using a spectrophotometer and a nano particle size analyzer. For comparison, use a centrifuge to test the stability of the microemulsion sample.The transmittance and particle size of the prepared microemulsion were measured and compared by using a spectrophotometer and a nanoparticle size analyzer. The orange oil microemulsion was then added to the cigarettes and subjected to artificial sensory evaluation; the smoke gas was analyzed by GC-MS. The experimental results showed that the final formulation of the sweet orange oil microemulsion was T-80: sucrose laurate = 9:1, the ratio between the surfactant and the cosurfactant Km=3:1, and the average particle size The diameter is only 20.16nm, and the light transmittance is above 95%. After centrifugation, the microemulsion remains uniform and stable, and there is no phenomenon of delamination and oil slick.There were significant differences in sensory attributes between cigarette added directly and microemulsions orange oil. A total of 155 aroma components were detected in the three types of cigarette samples. Among these substances, the more volatile compounds were heterocycles, ketones, and esters compounds.

Key Words: Orange oil，microemulsion，cigarette，sensory evaluation

目  录

1 绪论1

1.1 甜橙油介绍1

1.2微乳液介绍烟.1

1.3用香精香料介绍.1

1.4 卷烟烟气的分析方法2

1.5 卷烟的感官评定3

1.6 本课题的研究的目的和意义4

2 材料、主要设备和方法5

2.1 材料、试剂和样品信息5

2.2 主要仪器与设备5

2.3 实验方法6

2.3.1 甜橙油微乳液的制备6

2.3.2 甜橙油微乳液性质的测定7

2.3.3 甜橙油微乳应用于卷烟7

3 结果与讨论10

3.1 表面活性剂和助表面活性剂间比例确定10

3.2 表面活性剂的选择12

3.3 复配表面活性剂13

3.4 复配甜橙油微乳液性质的测定15

3.4.1 甜橙油微乳的分散相粒径分布的测定15

3.4.2 甜橙油微乳的透光率测定16

3.4.3 甜橙油微乳的稳定性测定17

3.5 卷烟加香分析17

3.5.1 烟气分析17

3.5.2 样品烟的感官评吸24

4.结论26

致  谢27

参考文献28

1 绪论

1.1 甜橙油介绍

甜橙特征香气物质很多，以具有挥发性的游离态芳香性物质和可转变成游离态的结合态芳香物质这两种形态。游离态芳香物质的化合物主要是脂类、醛类和萜烯类，萜烯糖苷是结合态芳香物质的主要化合物,可分解为游离态的芳香物质和糖。橙油中的芳香物广泛存在于橙皮的分泌组织中。绝大部分是对香气贡献较小的萜烯类化合物，含量不多的萜烯类氧化衍生物是甜橙香气的主要来源[1]。