摘要：水稻是我国重要的粮食作物，长江中下游地区是我国水稻主产区之一，而水稻分蘖期正值该地的暴雨多发期，容易引发淹涝胁迫，严重影响水稻生产。因此，研究淹涝胁迫对水稻生长的影响和伤害机理，提出相应的缓解技术减轻水稻涝害损失、提高淹后恢复能力具有重要意义。本研究以常规粳稻品种宁粳7号为材料，用不同浓度的BABA（β-氨基丁酸）（1、3、5mmol/L）处理解除淹水胁迫后的水稻，喷施清水作为对照，另设置一不淹水处理作为整个淹水处理的对照，研究叶面喷施BABA对受淹水稻生理生化特性的影响，探讨其对缓解水稻涝害的生理生化机制。结果表明叶面喷施BABA可以显著改善淹水胁迫对水稻的生长抑制和氧化损伤。能加快受害水稻的单株绿叶数和单株茎蘖数的增加，有效降低受淹水稻MDA含量，增强抗氧化酶的活性。在修复作用方面，3mmol/L的BABA 最为有效，最终产量最为接近不淹水的水稻。

关键词：水稻；分蘖期；淹水胁迫；BABA；作用机理

The effect of BABA (β-aminobutyric acid) on the recovery of submerged rice at the tillering stage

Abstract：Rice is an important food crop in our country and most of it is planted in the midstream and downstream of Yangtze River. However, the tillering stage of rice is at the time of heavy rainfall in the area, which is likely to cause submergence stress and seriously affect rice production. Therefore, it has an important meaning to research the influence of submergence stress on rice growth and the damage mechanism, and put forward the corresponding mitigation technology to reduce loss. In this study, the conventional japonica variety Ningjing No.7 was used as material, treated with different concentrations of BABA (β-aminobutyric acid) (1, 3, 5 mmol/L) after the rice de-submergence and sprayed with clear water as a control. A non-flooding treatment was set as a control for the entire flooding treatment. The effect of foliar spraying of BABA on the physiological and biochemical characteristics of submerged rice was studied, and its physiological and biochemical mechanisms to alleviate rice waterlogging were discussed. The results showed that foliar spraying of BABA could significantly improve the submergence stress on rice growth inhibition and oxidative damage. It can accelerate the increase of the number of green leaves per plant and the number of stems per plant of the affected rice, effectively reduce the MDA content of submerged rice, and increase the activity of antioxidant enzymes. In terms of repair effect, 3mmol/L BABA is the most effective, and its yield is the closest to non-flooded rice.

Key words: rice；tillering stage；waterlogging Stress；BABA；mechanism

目  录

摘要1

关键词1

Abstract…1

Key words1

引言…1

1 材料与方法…2

1.1 试验材料与设计 …2

1.1.1 试验设计 …2

1.1.2 淹水条件 …2

1.2 数据收集及测定项目…3

1.2.1 单株茎蘖数、单株绿叶数、地上部干重、根干重、可溶性总糖、淀粉3

1.2.2 叶绿素、H2O2、MDA、抗氧化酶…3

1.2.3 产量及产量构成3

1.4 数据处理与分析…3

2 结果与分析3

2.1 叶面喷施BABA对受淹水稻茎蘖数和绿叶数的影响… 3

2.1.1 单株茎蘖数3

2.1.2 单株绿叶数4

2.2 叶面喷施BABA对受淹水稻干物重积累的影响…4

2.3 叶面喷施BABA对受淹水稻叶片叶绿素含量的影响…5

2.4 叶面喷施BABA对受淹水稻渗透调节物质含量的影响6

2.4.1 可溶性总糖…6

2.4.2 可溶性蛋白…6

2.5 叶面喷施BABA对受淹水稻MDA含量的影响…6

2.6 叶面喷施BABA对受淹水稻抗氧化酶系统的影响7

2.7 叶面喷施BABA对受淹水稻产量及其构成的影响8

3 讨论8

致谢…9

参考文献10

BABA（β-氨基丁酸）对分蘖期受淹水稻的修复作用