摘要： 本课题主要研究了薤白有效成分的最佳提取工艺条件，以中药薤白为原料，对薤白进行酒精浸泡、超声震荡处理后，采用旋转蒸发仪浓缩出薤白提取物中的有效成分。在单因素实验的基础上，以薤白提取物自由基清除率为考察指标，考察浸泡酒精浓度、浸泡液固比、超声温度、超声时间等因素对薤白提取物抗羟基自由基氧化率的影响。并在此基础上对提取工艺进行优化，利用紫外分光光度计观测薤白提取物的紫外吸光度，对实验数据进行分析。结果表明：提取薤白有效成分的最佳工艺条件为浸泡酒精浓度75%、浸泡温度40℃、超声处理30min、液固比4.50∶1，此四种条件下薤白提取物的紫外吸光度最强，抗羟基自由基氧化率最高，即抗氧化性最强。而后将提取物应用到化妆品中，考察产品的稳定性、保湿性和功效性。对薤白中有效成分的保湿、抗氧化和抗菌等功效性进行研究检测。

关键词： 薤白；提取物；单因素；紫外吸光度；超声处理；旋蒸；抗氧化；化妆品

Extraction and Efficacy Evaluation and Application of Sophora chinensis

Abstract: This topic mainly studies the extraction of effective components from Radix Paeoniae Alba. After the Chinese herbal medicine was used as the raw material, the wolfberry white was subjected to alcohol soaking and ultrasonic vibration treatment, and then the extract in the indole white was extracted using a rotary evaporator. On the basis of single factor test, the UV absorbance of the extract was used as an index to investigate the effects of soaking alcohol concentration, soaking liquid solid ratio, ultrasonic temperature, and ultrasonic time on the ultraviolet absorbance of the extract of Sophora japonica. On this basis, the extraction process was optimized. Ultraviolet spectrophotometer was used to observe the UV absorbance of Radix Paeoniae extract and the experimental data was analyzed. The results showed that the optimum conditions for extracting the effective components of Radix Paeoniae Rubra were as follows: soaked alcohol concentration 75%, soaking temperature 40 °C, sonication 30 min, liquid-solid ratio 4.50∶1, and the UV absorbance of Radix Paeoniae Alba extract was the strongest. That is, the highest oxidation resistance. The extract was then applied to cosmetics to examine the stability and efficacy of the product. The moisture, anti-oxidation and antibacterial effects of the active ingredients in Radix Paeoniae Rubra are studied and tested.

Key words: Radix Paeoniae Alba;extract;single factor;ultraviolet absorbance;ultrasonic treatment;steam rot;oxidation;cosmetics

目录

1 前言 1

1.1 薤白介绍 1

1.1.1 薤白的化学成分 1

1.1.2 薤白的历史进展 2

1.1.3薤白的应用 2

1.2 薤白成分的提取技术研究 3

1.2.1 超声提取法 3

1.2.2 超临界CO2萃取 3

1.2.3 水溶液提取法 4

1.2.4 有机溶剂提取法 4

1.2.5 水蒸气蒸馏法 5

1.3 抗衰老化妆品的研究近况 5

1.3.1 皮肤抗衰老机理 5

1.3.2 抗衰老化妆品前景 5

1.4 研究目的和意义 6

1.4.1 研究目的 6

1.4.2 研究意义 6

2 实验方法及步骤 7

2.1 实验拟使用的原料试剂和实验设备 7

2.1.1 实验拟使用的原料试剂 7

2.1.2 实验拟使用的实验设备 7

2.2 实验方法及功效评价 8

2.2.1 化妆品的功效性能评价 8

2.2.2 化妆品的稳定性能评价 9

2.3 实验步骤 9

2.3.1 制备薤白的乙醇提取物 9

2.3.2 薤白提取物的超声处理 10

2.3.3 薤白提取物的离心处理 10

2.3.4 薤白提取物的抽滤旋蒸 10

2.3.5薤白提取物在基质膏霜中的应用 11

2.3.6 薤白提取物紫外吸光度的测定 12

2.3.7 薤白提取物功效性的测定 12

2.3.8 薤白提取物在化妆品中的稳定性的测定 13