摘要：益生菌和植物提取物作为抗生素的绿色替代品在畜牧业生产中越来越普遍的使用。本实验旨在通过体外培养嗜酸乳杆菌,比较其固液发酵体系的产物,以及研究不同浓度的植物提取物对益生菌体外增殖效果的影响，为发酵饲料的生产和植物提取物与益生菌的联合应用提供试验依据。试验利用固态发酵法和液态发酵法来比较不同发酵体系产生的乳酸，VFA，PH值及CFU的差异；用吸光光度法、平板计数法筛选嗜酸乳杆菌，将筛选出的嗜酸乳杆菌与植物提取物共培养。分析实验结果表明，固态发酵体系中，三株菌各有优势；液态发酵体系中，G菌和H菌优势作用明显；且不同浓度的植物提取物的增菌效果不同，当添加量2‰时对嗜酸乳杆菌生长的促进作用最显著。

关键词：嗜酸乳杆菌；植物提取物；发酵体系；协同作用

Comparison of fermentation systems of Lactobacillus acidophilus from three different strains and its synergies with plant extracts

Abstract：Probiotics and plant extracts as a sustainable alternative to antibiotics are more and more widely used in animal husbandry production.The aim of this experiment is through in vitro culture to compare different Lactobacillus acidophilus fermentation systems and to explore the effects of different concentrations of plant extracts on the in vitro proliferation of probiotics, providing the experimental basis for the production of fermented feed as well as the combined application of compound plant extracts and probiotics.The experiment compared the products of solid state fermentation system and liquid state fermentation system; then selected Lactobacillus acidophilus through absorbance spectrophotometry and flat counting method,and put the selected Lactobacillus acidophilus and plants extracts cultivated in vitro together.The results show that inthe solid state fermentation system, each of the three bacterial strains has its advantages;but in the liquid state fermentation,the predominant effects of G-bacilli and H-bacilli were obvious;and different concentration of plants extracts has different effects on Lactobacillus acidophilus, when the amount of addictive is 2‰,plants extracts has most significant effects on promoting proliferation of Lactobacillus acidophilus.

Key words: Lactobacillus acidophilus；plants extracts；fermentation systems；synergies

目  录

摘要1

关键词1

Abstract1

Key words1

引言（或绪论）1

实验一  嗜酸乳杆菌的固态发酵与液态发酵4

1 材料与方法4

1.2实验方法 5

1.3数据处理5

2结果与分析5

2.1固态发酵体系产物的变化5

2.1.1固态发酵体系PH变化5

2.1.2固态发酵体系菌落计数结果5 2.1.3固态发酵体系乳酸含量6

2.1.4固态发酵体系VFA含量6

2.2液态发酵体系产物的变化6      

2.2.1液态发酵体系PH变化6

2.2.2 液态发酵体系菌落计数结果 6

2.2.3液态发酵体系乳酸含量7

2.2.4液态发酵体系VFA含量7

实验二  不同浓度的复合提取物对嗜酸乳杆菌生长的影响7

1 材料与方法7

1.1实验材料 7

1.2实验方法 7

1.2.1 嗜酸乳杆菌的筛选7

1.2.2物提取物与嗜酸乳杆菌共培养7

1.3测定指标7

1.4数据处理8

2结果与分析8

3讨论9

3.1 嗜酸乳杆菌固液发酵体系的比较 9

3.2植物提取物对嗜酸乳杆菌生长的影响  10

致谢11

参考文献11

三株不同来源的嗜酸乳杆菌发酵体系的比较及其与植物提取物的协同作用

引言

自二十世纪中叶发现了抗生素的治病抗病及提高动物生产性能的功效以来，动物饲粮中添加抗生素作为生长促进剂得到广泛应用，极大的促进了畜产品的集约化生产。然而随着时代的发展,滥用抗生素的危害越来越凸显。欧盟在2006年颁布了禁止在动物饲料中使用抗生素的法令，目前，动物饲料中不得添加抗生素已经成为社会各界关注的焦点。因此急需能够代替抗生素功能又符合安全、可持续发展理念的替代品来保障畜牧业生产的效益，科学家们开始将科研方向转为植物提取物和益生菌的研究。植物提取物作为一种抗生素的绿色替代品在畜禽饲料中得到越来越广泛的应用其，具备安全性、高效性、无抗残留、不产生抗药性等优势，可大大减少滥用抗生素带来的危害。