摘要：荷花（Nelumbo nucifera）是重要的水生草本花卉，常常被用于园林水体绿化；近年来研究表明荷花还可以作为植物修复材料用于水体的净化，但是关于荷花的重金属耐性机制仍不清楚。本课题以研究荷花耐镉（Cd）机理为出发点，考虑到乙烯与糖信号间存在广泛及复杂的交叉互作，主要探索乙烯及葡萄糖会对Cd胁迫下荷花幼苗的生长产生怎样的影响。本试验首先通过采用乙烯前体ACC与乙烯作用抑制剂STS处理证实了乙烯参与调控Cd胁迫对荷花幼苗生长的抑制。在此基础上，通过葡萄糖、甘露醇处理，以及葡萄糖或甘露醇与ACC的复合处理后，测定不同处理下的形态指标、电导率、MDA含量以及抗氧化系统的变化来探究葡萄糖信号与乙烯互作在调控荷花响应Cd胁迫中的作用。研究结果发现, Cd胁迫会抑制荷花幼苗生长，外源乙烯会加重抑制作用，葡萄糖能够通过拮抗乙烯作用进而减缓Cd胁迫对荷花幼苗产生的抑制作用，提高荷花幼苗POD、CAT、APX和GR的活性和抗氧化剂AsA、GSH的含量，从而提高荷花幼苗抗氧化能力和抗逆性。

关键词：荷花幼苗，镉胁迫，葡萄糖，乙烯

Effect of Ethylene and Glucose on the Lotus Response to Cadmium Stress

Abstract：Lotus（Nelumbo nucifera）is an important aquatic herb, often used for landscaping in gardens. Recent studies have shown that lotus can also be used as a plant repair material for water purification, but the mechanism for heavy metal tolerance was still unclear. This topic used studying lotus cadmium(Cd) resistant mechanism as a starting point, considering the ethylene and sugar signal cross interactions between the extensive and complex, mainly explore the ethylene and glucose on the growth of the lotus seedlings under Cd stress will produce what kind of impact. This experiment was first used to confirm the inhibition of ethylene involvement in the growth of lotus seedlings by the use of the ethylene precursor ACC and the vinyl action inhibitor STS. On this basis, the treatment of glucose, mannitol, glucose or mannitol and ACC composite processing, measuring indexes under

different processing, electric conductivity, MDA content and the change of antioxidant system to explore glucose signal with ethylene role in regulating the lotus Cd stress response. The results of the study showed that Cd stress suppresses lotus seedlings growth, exogenous ethylene can aggravate the inhibition, glucose could through the antagonism of ethylene and slow the inhibitory effect of Cd stress on the lotus seedlings, increase the lotus seedlings POD, CAT, APX and GR activity and the antioxidant content of AsA and GSH, thus improve the lotus seedlings antioxidant capacity and resistance.

Key words: lotus seedlings；cadmium stress；glucose；ethylene

目 录

摘要1

关键词1

Abstract1

Key words2

引言2

1材料与方法3

1.1试验材料 3

1.2试验方法 3

1.2.1种苗培养 3

1.2.2幼苗形态观测3

1.2.3 相对电导率（EL）和丙二醛（MDA）含量的测定3

1.2.4 抗氧化酶活性测定3

1.2.5 抗氧化剂含量的测定4

1.2.6 统计分析4

2 结果与分析 4

2.1乙烯对荷花幼苗响应镉胁迫的影响4

2.1.1 幼苗形态的差异比较4

2.1.2 相对电导率（EL）和丙二醛(MDA)含量差异分析5

2.1.3 抗氧化酶活性差异分析6

2.1.4 抗氧化剂含量差异分析7

2.2 葡萄糖对荷花幼苗响应镉胁迫的影响 7

2.2.1幼苗形态的差异比较 7

2.2.2 相对电导率（EL）丙二醛（MDA）含量差异分析8

2.2.3 抗氧化酶活性差异分析9

2.2.4抗氧化剂含量差异分析10

3 讨论11

3.1 乙烯对荷花幼苗响应镉胁迫的影响11

3.2葡萄糖对荷花幼苗响应镉胁迫的影响11

4 结论11

致谢12

参考文献12

乙烯、葡萄糖对荷花响应镉胁迫的影响